We are seeking a highly motivated Ph.D. student to address how autophagic vesicle trafficking is controlled in noradrenergic LC neurons.
The project requires expertise in molecular cloning, projection-specific labeling of LC neurons with retro- and anterograde viruses, Crispr/Cas manipulations, Two-Photon in-vivo imaging. We will use advanced image processing to segment axonal trajectory in cleared brain tissues. Confocal and Super-resolution STED imaging, as well as light-sheet microscopy.
The suitable candidate should have a general background in cell biology and physiology. S/He should be interested in state-of-the-art optical imaging techniques and employing novel optogenetic tools; this project relies on working with mice, and a FELASA certificate is beneficial as well as having a good understanding of molecular aspects and methodology. Previous experience in one of the techniques mentioned above is a plus but not a prerequisite.
We offer a stimulating environment in an international and dynamic team. Equal opportunities for women and men are ensured.
Applications should include a cover letter stating research interests and previous experience, a CV, and contact information of two referees. Correspondence and inquiries should be sent to Dr. Anna Karpova (firstname.lastname@example.org) and Dr. Matthias Prigge (email@example.com)
Master Students / Rotation students
We are welcoming application from master students or students interest in doing a rotation in our lab. We will offer experience in various state-of-the-art techniques in our lab:
- mouse behavior + opto/chemogenetics
- electrophysiology (patch clamp in vitro acute slices and multi-electrode array for single unit/LFP recording in vivo)
- 1-photon & 2-photon calcium imaging and optogenetic stimulation
- tissue clearing and viral tracing
- protein and photoreceptor engineering
There are many interesting master projects in our lab. Nonetheless, you are welcome to bring in your own ideas and discuss them with us. A reading list with a bundle of pdf to get to know our research and methodology can be requested via email.
Please feel free to contact firstname.lastname@example.org